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alphascreen plate  (Revvity)


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    Structured Review

    Revvity alphascreen plate
    Alphascreen Plate, supplied by Revvity, used in various techniques. Bioz Stars score: 96/100, based on 13995 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/alphascreen+assay/EnVision+XCite+multimode+plate+reader/us12275725-394-1-3
    Average 96 stars, based on 13995 article reviews
    alphascreen plate - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Amplified Luminescent Proximity Homogenous Assay:

    Article Title: Identification of novel Plasmodium vivax proteins associated with protection against clinical malaria
    Article Snippet: .. Total IgG antibody measurements were made using the AlphaScreen assay following the manufacturer’s instructions (PerkinElmer Life and Analytical Sciences, Boston, MA) and as previously described ( ). .. Briefly, reactions were carried out in 384-well microtiter plates at 26 °C using a JANUS Automated Workstation (PerkinElmer).

    Article Title: Cell-free formation and interactome analysis of caveolae
    Article Snippet: After 2 h of incubation at 27°C, the 4-μl aliquot of reactions were subjected to SDS PAGE and visualized on a ChemiDoc XRS+ system (Biorad) using 488-nm excitation source and 520-nm detector. .. The AlphaScreen Assay was performed using the cMyc detection kit (Perkin Elmer) as previously described ( ). .. Samples were subjected to a fourfold serial dilution in buffer A (25 mM Hepes and 50 mM NaCl) and 2 μl of diluted samples were aliquoted into Proxiplate-384 Plus plate (Perkin Elmer) with 12.5 μl (0.4 μg) of anti-cMyc–coated acceptor beads in buffer B (25 mM Hepes, 50 mM NaCl, 0.001% vol/vol casein, and 0.001% vol/vol Nonidet P-40), and then, 2 μl biotin-labeled GFP nanobody in buffer A was added to make up a final concentration of 2.5 nM, followed by incubation for 45 min at RT.

    Article Title: ASGCT Annual Meeting Abstracts
    Article Snippet: TP was produced in BL21 E.coli as a thioredoxin fusion protein (TP-TRx) with 6-His tag, then purified using a HisTrap column (GE Healthcare) with in situ refolding on column. .. The TP fusion protein was tested using an Alphascreen assay (PerkinElmer) to probe for interactions with nuclear importins. .. Subsequently TP was labelled using maleimide-Alexa Fluor 594 and microinjected into the cytoplasm of HeLa cells (Injectman, Eppendorf).

    Article Title: Crystal structure of [1,2,4] triazolo[4,3- b ]pyridazine derivatives as BRD4 bromodomain inhibitors and structure–activity relationship study
    Article Snippet: BRD4 BD1 was concentrated to 12 mg/mL, which was measured by nanodrop, converted with the calculated extinction coefficient, and stored at −80 °C until crystallization. .. The AlphaScreen assay was described previously and performed according to the manufacturer’s protocol (PerkinElmer, USA). ..

    Article Title: Demonstrating Ligandability of the LC3A and LC3B Adapter Interface.
    Article Snippet: Autophagy is the common name for a number of lysosome-based degradation pathways of cytosolic cargos.. The key components of autophagy are members of Atg8 family proteins involved in almost all steps of the process, from autophagosome formation to their selective fusion with lysosomes.. In this study, we show that the homologous members of the human Atg8 family proteins, LC3A and LC3B, are druggable by a small molecule inhibitor novobiocin.

    Article Title: Protein–Protein Interactions in Plain View
    Article Snippet: PPIs are highly specific electrostatic attractions between protein structures.. These attractions may occur within individual proteins, resulting in globular structures, or between multiple proteins, resulting in complexes or aggregates.. By determining whether structures or complexes that participate in cell signaling stay together or fall apart, PPIs regulate cell function.

    Article Title: Discovery of a novel 53BP1 inhibitor through AlphaScreen-based high-throughput screening
    Article Snippet: Tumor suppressor p53-binding protein 1 (53BP1), a tantem tudor domain (TTD) protein, takes part in DNA Damage Repair (DDR) pathways through the specific recognition of lysine methylation on histones.. The dysregulation of 53BP1 is closely related to the development of many diseases including cancer.. Moreover, recent studies found that deficiency of 53BP1 could increase the efficiency of precise CRISPR/Cas9 genome editing.

    Protein-Protein interactions:

    Article Title: Protein–Protein Interactions in Plain View
    Article Snippet: PPIs are highly specific electrostatic attractions between protein structures.. These attractions may occur within individual proteins, resulting in globular structures, or between multiple proteins, resulting in complexes or aggregates.. By determining whether structures or complexes that participate in cell signaling stay together or fall apart, PPIs regulate cell function.

    Fluorescence:

    Article Title: Protein–Protein Interactions in Plain View
    Article Snippet: PPIs are highly specific electrostatic attractions between protein structures.. These attractions may occur within individual proteins, resulting in globular structures, or between multiple proteins, resulting in complexes or aggregates.. By determining whether structures or complexes that participate in cell signaling stay together or fall apart, PPIs regulate cell function.

    Spectroscopy:

    Article Title: Protein–Protein Interactions in Plain View
    Article Snippet: PPIs are highly specific electrostatic attractions between protein structures.. These attractions may occur within individual proteins, resulting in globular structures, or between multiple proteins, resulting in complexes or aggregates.. By determining whether structures or complexes that participate in cell signaling stay together or fall apart, PPIs regulate cell function.

    Amplification:

    Article Title: Protein–Protein Interactions in Plain View
    Article Snippet: PPIs are highly specific electrostatic attractions between protein structures.. These attractions may occur within individual proteins, resulting in globular structures, or between multiple proteins, resulting in complexes or aggregates.. By determining whether structures or complexes that participate in cell signaling stay together or fall apart, PPIs regulate cell function.

    Imaging:

    Article Title: Protein–Protein Interactions in Plain View
    Article Snippet: PPIs are highly specific electrostatic attractions between protein structures.. These attractions may occur within individual proteins, resulting in globular structures, or between multiple proteins, resulting in complexes or aggregates.. By determining whether structures or complexes that participate in cell signaling stay together or fall apart, PPIs regulate cell function.



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    Image Search Results


    The small molecule compound NSC617570 suppresses the interaction between PRKCQ‐AS1 RNA and MSI2 protein and shows anticancer effects in vitro and in vivo. A) PRKCQ‐AS1 RNA fragment 1 (65‐306 bp at the 5′‐ end) was in vitro transcribed, biotin‐labelled, mixed with Flag‐tagged MSI2 protein, and subjected to AlphaScreen of 2932 small molecule compounds at 10 µM for inhibitors of the interaction between PRKCQ‐AS1 RNA and MSI2 protein. Eight‐five compounds were found to reduce the interaction between PRKCQ‐AS1 RNA and MSI2 protein by > 70% (above the red line). B) Structure of the “hit” compound NSC617570. C) SK‐N‐AS cells were treated with vehicle control or 10 µM NSC617570 for 6 h, followed by RNA immunoprecipitation assays with control IgG or MSI2 antibody and RT‐PCR with primers targeting PRKCQ‐AS1 or BMX. Data were shown as the mean ± standard deviation of three independent experiments and evaluated by Student's t ‐test. * indicated p < 0.05. D‐E) SK‐N‐AS and SK‐N‐SH cells were treated with vehicle control, 5 µM or 10 µM NSC617570 for 48 h, followed by RT‐PCR analysis of BMX mRNA (D) and immunoblot analysis of BMX protein, total ERK protein (ERK) and phosphorylated ERK protein (phos‐ERK) (E). F) SK‐N‐AS and SK‐N‐SH cells were treated with vehicle control or a range of doses of NSC617570 for 72 h, followed by Alamar blue assays. Data were shown as the mean ± standard deviation of three independent experiments and evaluated by ANOVA. *, ** and *** indicated p < 0.05, 0.01 and 0.001 respectively. G) SK‐N‐AS and SK‐N‐SH cells were treated with vehicle control, 5 µM or 10 µM NSC617570 for 14 (SK‐N‐AS) or 21 (SK‐N‐SH) days, followed by clonogenic assays. H‐I) SK‐N‐AS cells were xenografted into nude mice. When tumors reached 50 mm 3 , the mice were treated with NSC617570 at 8.75 mg/kg or vehicle control via i.p. injection, 5 times per week. Tumor growth was monitored (H) and mouse overall survival was analyzed (I). For survival analysis, P value was obtained from two‐sided log‐rank test.

    Journal: Advanced Science

    Article Title: The Super Enhancer‐Driven Long Noncoding RNA PRKCQ‐AS1 Promotes Neuroblastoma Tumorigenesis by Interacting With MSI2 Protein and Is Targetable by Small Molecule Compounds

    doi: 10.1002/advs.202412520

    Figure Lengend Snippet: The small molecule compound NSC617570 suppresses the interaction between PRKCQ‐AS1 RNA and MSI2 protein and shows anticancer effects in vitro and in vivo. A) PRKCQ‐AS1 RNA fragment 1 (65‐306 bp at the 5′‐ end) was in vitro transcribed, biotin‐labelled, mixed with Flag‐tagged MSI2 protein, and subjected to AlphaScreen of 2932 small molecule compounds at 10 µM for inhibitors of the interaction between PRKCQ‐AS1 RNA and MSI2 protein. Eight‐five compounds were found to reduce the interaction between PRKCQ‐AS1 RNA and MSI2 protein by > 70% (above the red line). B) Structure of the “hit” compound NSC617570. C) SK‐N‐AS cells were treated with vehicle control or 10 µM NSC617570 for 6 h, followed by RNA immunoprecipitation assays with control IgG or MSI2 antibody and RT‐PCR with primers targeting PRKCQ‐AS1 or BMX. Data were shown as the mean ± standard deviation of three independent experiments and evaluated by Student's t ‐test. * indicated p < 0.05. D‐E) SK‐N‐AS and SK‐N‐SH cells were treated with vehicle control, 5 µM or 10 µM NSC617570 for 48 h, followed by RT‐PCR analysis of BMX mRNA (D) and immunoblot analysis of BMX protein, total ERK protein (ERK) and phosphorylated ERK protein (phos‐ERK) (E). F) SK‐N‐AS and SK‐N‐SH cells were treated with vehicle control or a range of doses of NSC617570 for 72 h, followed by Alamar blue assays. Data were shown as the mean ± standard deviation of three independent experiments and evaluated by ANOVA. *, ** and *** indicated p < 0.05, 0.01 and 0.001 respectively. G) SK‐N‐AS and SK‐N‐SH cells were treated with vehicle control, 5 µM or 10 µM NSC617570 for 14 (SK‐N‐AS) or 21 (SK‐N‐SH) days, followed by clonogenic assays. H‐I) SK‐N‐AS cells were xenografted into nude mice. When tumors reached 50 mm 3 , the mice were treated with NSC617570 at 8.75 mg/kg or vehicle control via i.p. injection, 5 times per week. Tumor growth was monitored (H) and mouse overall survival was analyzed (I). For survival analysis, P value was obtained from two‐sided log‐rank test.

    Article Snippet: AlphaScreen assays were performed using purified human recombinant MSI2 protein (TP301003, Origene Technologies, Rockville, MD) and PRKCQ‐AS1 RNA fragment 1 (306 base pairs).

    Techniques: In Vitro, In Vivo, Amplified Luminescent Proximity Homogenous Assay, Control, RNA Immunoprecipitation, Reverse Transcription Polymerase Chain Reaction, Standard Deviation, Western Blot, Injection